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The Science Behind Firefly Dx

The Firefly Dx system is designed to operate using real-time polymerase chain reaction (PCR) chemistry. PCR amplifies a sample’s DNA and copies it billions of times. PCR is used every day to diagnose diseases, identify bacteria and viruses, and for many other applications. With real-time PCR, the amplification of the DNA can be measured as it occurs, thereby speeding up the ability to deliver results. Real-time PCR is one of the most sensitive and specific bio-detection capabilities available today.

A critical and highly complicated step prior to performing real-time PCR is sample preparation. ExcitePCR’s patented sample preparation technologies use ultrasonic lysis for the rupture of tough cellular membranes. The lysed sample is then microfluidically passed through a purification apparatus that isolates and purifies nucleic acid. The Company’s expertise in sample prep is reflected by its strong intellectual property position.